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DeCS
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Descriptor English:
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Freeze Etching
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Descriptor Spanish:
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Grabado por Congelación
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Descriptor Portuguese:
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Técnica de Congelamento e Réplica
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Synonyms English:
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Etching, Freeze
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Tree Number:
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E01.370.225.500.620.620.260.400
E01.370.225.750.600.620.260.400
E05.200.500.620.620.260.400
E05.200.750.600.620.260.400
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Definition English:
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A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces. |
Indexing Annotation English:
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do not confuse with FREEZE FRACTURING (wherein a cryoprotectant is used)
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History Note English:
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73(69)
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Allowable Qualifiers English:
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Record Number:
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5749
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Unique Identifier:
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D005613
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Occurrence in VHL:
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Similar:
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DeCS
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